Wavelength: 275nm Flow rate: 1.0ml / min

Mobile phase: methanol: buffer = 150: 850

Buffer: 500ml water + 10ml glacial acetic acid + 1.3ml triethylamine + 20ml sodium hexanesulfonate (0.005mol / L), add water to 1000ml,

Adjust the pH to 3.2

Column: Chenhang Column 88181545 C18 5μ 150 × 4.6 811060

Instrument used: CoMetro 6000 high performance liquid chromatograph

--6000 LDI precision constant flow pump

--6000 PVW UV detector

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