Serum troponin T immunoradiology diagnostic kit
cTnt (HY-49) IPMA KIT instruction manual
Clinical significance:
Cardiac troponin T (cTnt) is one of the components of cardiac troponin complex. It and TnT of skeletal muscle are derived from different gene codes and have different protein structures, so they have high immunospecificity. There are two main forms of CTnt in the myocardium, about 5% is soluble cTnt located in the cytoplasm. Because of its small molecular weight (about 37.000), cTnt enters the blood circulation quickly after AMI occurs, and the average increase time It is 3 hours, the range is 1-10 hours, and the first peak appears in the blood about 12-24 hours after the onset. Therefore, cTnt is an early sign of AMI. The remaining cTnt in the myocardium exists in the form of structural proteins. During AMI, it gradually degrades and releases into the blood, forming a second peak of cTnt in the blood, which increases the duration of cTnt in the blood to 7 days. During the diagnosis window period, the specificity of diagnosing AMI during this diagnosis period is close to 100%. In addition, the content of cTnt in normal blood is very low (less than 0.1ng / ml), and the concentration of cTnt in the blood increases at least several times when AMI occurs, so this diagnostic indicator has high sensitivity. Because of its high myocardial specificity, high sensitivity, early diagnosis and wide window period, cTnt has been considered as a near-ideal and versatile marker of myocardial infarction.
Method and principle:
The kit uses the double antibody sandwich one-step reaction principle to detect cardiac troponin T (cTnt) in serum. The monoclonal anti-troponin T antibody coated on the polyethylene tube binds to the sample or standard troponin T, and another 125I-monoclonal anti-troponin T antibody binds to the coated antibody at the same time Troponin T combines to form a monoclonal anti-troponin T antibody-troponin T-125I monoclonal anti-troponin T antibody complex, which can be processed by measuring the CPM value of the standard tube and sample tube and the corresponding processing mode. Calculate the content of troponin T in the sample to be tested.
Sample handling: Separate serum as soon as possible after blood draw to avoid hemolysis. The sample should be tested within 6 hours, otherwise it should be frozen at -20 ℃, but repeated freezing and thawing should be avoided.
Kit composition:
Product specification
Product composition
Number of components in each box
Remarks
50 tubes
Coating tube (50 tubes / bag)
1 bag
Keep sealed after use
Standard (S0-S5, the concentrations are: 0, 0.08, 0.4, 1.2, 3.2, 16.0ng / ml,
Before use, add 0.25ml of normal saline to each bottle to dissolve)
3 sets
Ready to use
125I monoclonal anti-troponin T antibody (5ml / bottle)
1 bottle
Operation process:
Take a number of coated tubes, add 200ul each of the standard samples to be tested after the number, and then add 50ul of the labeled substance wealth, shake at room temperature for 1.5 hours after mixing, drain the reaction solution, add at least 3ml of distilled water to each tube, and repeat 4 times . Gamma counter measures for 1 minute, and it is possible to determine the content of troponin T in the sample to be measured after using the IRMA program or calculator of the radioimmunoassay. A laboratory that uses a calculator to process data can use Casil-3600P, using a double logarithmic processing mode, and SO counts should be deducted from S1 to S5 before input. The laboratory used for scientific research can adopt the reaction conditions of room temperature overnight.
Precautions:
1. Before equilibration to room temperature.
2. Pay attention to avoid contamination of the pipe wall when adding samples.
3. The storage time of the sample to be tested at 2-8 ℃ shall not exceed 6 hours.
4. The standard is used as it is and it should not be stored at room temperature.
Product Technical Index:
1. Sensitivity: <0.05ng / ml.
2. The cross reaction with skeletal troponin and myocardial protein I is less than 0.01%.
3. Precision: CV within the batch <10%, CV between batches <15%.
Normal value: The measured values ​​of 126 normal blood samples are:
Range: 0ï¼0.05ng / ml;
Mean 0.03 ng / ml:
SD = 0.01.
The normal value is determined to be less than 0.1 ng / ml.
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