With age, hyaluronic acid/collagen in the skin disappears and the result is a loss of freshness and freshness of the skin.With Reborn PLLA Hydrogel, the skin's collagen is regenerated so that the skin's tissues are well aligned and the skin is rejuvenated and beautiful.Reborn Liquid Filler 5ml in vial is liquid PLLA for skin improvement solution. Mainly for fine wrinkles and facial texture improvement.Reborn PLLA Liquid Filler can be used by hand needle, mesogun,microneedle and other device.
What is special about hydrogel?
Freeze-dried PLLA does not contain particles. Hence the absence of adverse reactions.
What effects can be achieved?
There are a lot of them~~~
Moisturizing, lightening, regeneration of scars and post-acne, narrowing pores, increasing skin density and elasticity, getting rid of rosacea, etc..
How quickly will the result be?
Lightening and moisturizing of the skin occurs after 5-7 days. The effect of skin flap tightening is after 3 weeks.
IMPORTANT!
- Injection of the gel only intradermally using a 27-32 g needle. Kolem pointwise. If you inject into the hypodermis, you will get severe swelling.
- Volume on the face - up to 1.5 ml, on the hands - 1 ml for each hand
- Course - 3 procedures with an interval of once every 3-4 weeks. Further maintenance procedures are recommended every 3-4 months.
Reborn Plla Hydragel,Face Scar Removal Plla Gel,Skin Rejuvenation Treatments Plla Hydragel,Plla Hydrogel Remove Neck Wrinkles Rimless Industry Co.,Ltd. , https://www.rebornplla.com
**Rat E-Cadherin (E-cad) ELISA Kit – Instructions for Use**
This kit is intended for research purposes only and is designed to quantify E-cadherin (E-cad) levels in rat serum, plasma, and related biological fluids. The assay is based on the double-antibody sandwich ELISA method. A solid-phase antibody specific to E-cad is pre-coated onto microwells. After adding the sample, E-cad in the sample binds to the coated antibody. An HRP-conjugated secondary antibody then recognizes the captured E-cad, forming a complex. Following washing steps, TMB substrate is added, which turns blue under HRP activity and then yellow when an acid stop solution is applied. The intensity of the color is directly proportional to the E-cad concentration in the sample. Absorbance is measured at 450 nm using a microplate reader, and concentrations are determined by comparing the sample OD values to a standard curve.
**Kit Components**
The kit includes:
- Microtiter plate (48 or 96 wells)
- Standard (180 ng/L)
- Standard diluent
- Enzyme-labeled reagent
- Sample diluent
- TMB substrate (A and B solutions)
- Wash buffer (concentrated, 20x or 30x)
- Sealing film and bag
**Storage Conditions**
All components should be stored at 2–8°C. The kit has a shelf life of 6 months from the date of manufacture.
**Sample Preparation Guidelines**
- **Serum:** Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes.
- **Plasma:** Use EDTA or sodium citrate as anticoagulant. Mix well, then centrifuge similarly.
- **Urine:** Centrifuge at 2000–3000 rpm for 20 minutes.
- **Cell culture supernatant:** Centrifuge at 2000–3000 rpm after collection. For intracellular components, lyse cells via freeze-thaw cycles before centrifugation.
- **Tissue samples:** Homogenize in PBS (pH 7.4), centrifuge, and collect the supernatant.
**Important Notes**
- Avoid repeated freezing and thawing of samples. Store at -20°C if not used immediately.
- Do not use samples containing NaN3, as it may inhibit HRP activity.
- Always prepare a standard curve with duplicate wells. If the sample OD exceeds the highest standard, perform a preliminary dilution.
- Ensure all reagents are at room temperature before use.
- Handle all waste materials as biohazardous.
**Procedure Summary**
1. Prepare standards by serial dilution.
2. Add sample and standards to the plate.
3. Incubate at 37°C for 30 minutes.
4. Wash the plate 5 times with diluted wash buffer.
5. Add enzyme-labeled reagent and incubate again.
6. Add TMB substrate and develop color for 15 minutes.
7. Stop the reaction with the stop solution.
8. Measure OD at 450 nm.
**Data Analysis**
Plot the standard curve using concentration vs. OD values. Calculate the sample concentration using linear regression or direct comparison. Multiply by the dilution factor to obtain the actual value.
**Performance**
- Correlation coefficient (R) ≥ 0.92
- Intra-assay CV < 9%, Inter-assay CV < 15%
- Detection range: 3 ng/L – 150 ng/L
**Safety & Compliance**
Follow all safety guidelines and use personal protective equipment. This manual is the official guide; any discrepancies with other language versions shall be resolved by the English version.
**Note:** Always refer to the latest version of the manual for updates and additional information.