Rust Color Corten steel Flower Planter
Corten steel flower pots&planter is made of corten steel,Which can be used to plant different kinds of flowers and widely used in garden. With the cone shape and simple design, it brings a new touch of style to your home or garden. Corten Steel Planter is designed in simple but practical, which is popular in Australia and European countries.
Packing
Packing will by pallet/carton/ wooden box,according to different demand.
Corten Steel Flower Planter,Garden Plant Pots,Flower Pot Holder,Metal Plant Pots Henan Jinbailai Industrial Co.,Ltd , https://www.gardensteelarts.com
Our Corten steel planters are made using high quality corten steel. Specifications include 2.0mm thick corten steel on the side panels, laser cut, folded, welded and dressed.
Name
LRust Color Corten steel flower planter
Material
Corten steel
Size
1500*500*600mm or customized
Steel thickness
2mm
Packing
Pallet/carton/wooden box packing
Q: I'm looking for information on the detection of acetylcholinesterase (AChE) on red blood cell membranes and methods for preserving blood. If you have any knowledge on this, please get in touch.
A: AChE is an enzyme found on the surface of red blood cells. It catalyzes the breakdown of acetylcholine into choline and acetate. In some assays, this reaction is coupled with a colorimetric method where DPIP (2,6-dichlorophenolindophenol) acts as an electron acceptor. As DPIP is reduced to a colorless form, the change in absorbance at 600 nm can be measured to determine AChE activity. This method has been used on a Hitachi 7060 bioanalyzer, with reference values for 85 healthy individuals ranging from 46.6 to 61.6 U/gHb. The intra-assay coefficient of variation was between 2.23% and 2.66%, while the inter-assay CV ranged from 4.31% to 4.78%. The sensitivity of the test is 0.015 U/gHb. This technique allows continuous monitoring, is not affected by hemolysis or bilirubin, and is well-suited for automated clinical testing.
Answer: Blood preservation has evolved significantly over time. Initially, blood was transfused immediately after collection, with no need for storage. However, modern practices involve storing blood in blood banks for later use. To ensure that the blood remains viable when administered, it's crucial to maintain the function and survival of red blood cells during storage. This involves addressing various challenges, such as cell damage due to storage conditions. The key factors include the type of blood container, anticoagulants, and preservation solutions, with the latter two being particularly important.
One major issue during storage is the degradation of red blood cells. Over time, they undergo biochemical and structural changes, known as storage lesions. These changes reduce their ability to function effectively after transfusion. A key indicator of viability is the percentage of red blood cells surviving 24 hours post-transfusion. Ideally, this should be above 70%.
ATP depletion is one of the most significant changes observed during storage. ATP breaks down into ADP and AMP, which further degrades into IMP. This leads to a reduction in the nucleotide pool. Red blood cells can regenerate ATP using adenine and ribose-5-phosphate, which is why some preservation solutions include adenine. However, recent studies suggest that other factors, such as membrane instability and shape changes, may play a more critical role in determining cell survival.
Red blood cells can also lose their biconcave shape and become spherical, leading to loss of membrane integrity. While early changes may be reversible, severe deformation is often irreversible and reduces cell viability. Membrane stability is also influenced by the materials used in blood bags, such as those containing DEHP, though its long-term effects are still under investigation.
Anticoagulants like citrate are widely used to prevent clotting during storage. Citrate binds calcium ions, inhibiting coagulation, and is metabolized by the body. It also helps prevent hemolysis. Heparin, another anticoagulant, is less favorable because it doesn't support red blood cell metabolism and leads to faster ATP loss, reducing cell viability. Heparin is typically used within 48 hours of collection and is now less common due to advances in component transfusion.
Blood preservation solutions, such as ACD and CPD, contain citrate, glucose, phosphate, and adenine. CPD includes adenine and phosphate, extending storage up to 35 days and improving oxygen release. ACD, without these additives, only supports storage for 21 days. Glucose in the solution provides energy for red blood cells, helping to prolong storage and prevent hemolysis.
Modern blood components, like concentrated red blood cells, require specific preservation solutions, and cryopreservation is sometimes used. Platelets, on the other hand, are best stored at room temperature (around 22°C) to maintain their function.