Heat transfer machine is a heat transfer technology used in the general term of the machine, heat transfer machine, including flat heat press machine, high pressure heat press machine, shaking his head heat press machine, baking cup machine, baking machine, baking machine and other thermal transfer machine. Can print a wide range of both flat heat transfer, including cups, plates, hats, these surface heat transfer, thermal transfer is a new technology, just just on the right track.
Heat Transfer Machine Heat Transfer Machine,Roller Garment Heat Transfer Machine,Plate Heat Transfer Machine,Heat Transfer Film Printing Machine KC Printing Machine (Group) Limited , http://www.kcautopm.com
Heat Transfer Machine
Features of heat transfer process
Advantage
1. The steps are simple
It does not need to plate, copy, repeat the chromatography step, without the need for screen printing and thermal transfer methods required for various types of tools, materials. With a universal printer, you only need to prepare a separate computer. A computer operator can be completely independent of the printing operation, the province of human and material resources, and the way is simple, Lidengkequ, the operator of the experience of low requirements, as long as the understanding of a simple picture processing software on it.
2. To avoid damage
It can be printed not only on the tough crystal, stone, metal, glass and other materials, but also can be printed on the soft texture of leather, cloth, cotton and other materials; it can be printed on the inorganic, can also be printed on the composition of complex , Change the organic matter on. On the material with more and better compatibility, the use of spring-hui of the number of straight-jet printing machine to avoid the screen printing, water transfer printing material selection issues, but also to avoid the thermal transfer of leather, fabrics, cotton and other organic materials Destruction of the problem. It caters to the diversified needs of the market, to better provide users with more comprehensive production services.
3. Avoid skewing
Universal printer is no longer in the traditional printing mode and method is no longer a simple manual operation and technology printing, and computer technology and high content of computer synthesis and automatic control technology for a better organic combination can be very precise alignment Need to print the area and location, to avoid the manual printing encountered by the position of the problem. Because it is a one-time multi-color printing, there will be no problem of chromatic alignment. These advantages can also be engraving, etching very effective combination in the engraving of the region to print a beautiful picture, or after printing accurate etching, etc., in the carving industry can achieve a good breakthrough.
Atrial Natriuretic Peptide (ANP) ELISA Kit Instructions This reagent is for research use only: This kit is used to determine the content of central natriuretic peptide (ANP) in human serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human atrial natriuretic peptide (ANP) in the specimen. A microplate was coated with purified human atrial natriuretic peptide (ANP) antibody to make a solid-phase antibody, and atrial natriuretic peptide (ANP) was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled atrial natriuretic peptide (ANP) The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the atrial natriuretic peptide (ANP) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human atrial natriuretic peptide (ANP) in the sample was calculated by a standard curve. Kit composition: kit composition 48 well configuration 96 well configuration storage instructions 1 copy 1 copy sealing film 2 pieces (48) 2 pieces (96) 1 sealed bag 1 enzyme coated plate 1 × 48 1 × 96 2 Store standard at -8 ℃: 450ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ℃ store standard dilution 1.5ml × 1 bottle 1.5ml × 1 bottle 2-8 ℃ store enzyme label reagent 3 ml × 1 bottle of 6 ml × 1 bottle of sample diluent at 2-8 ° C 3 ml × 1 bottle of 6 ml × 1 bottle of 2-8 ° C Developer A 3 ml × 1 bottle of 6 ml × 1 bottle of 2-8 ° C Developer B solution 3 ml × 1 bottle 6 ml × 1 bottle at 2-8 ° C storage stop solution 3ml × 1 bottle 6ml × 1 bottle at 2-8 ° C Concentrated washing solution (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle at 2-8 ° C to store the sample. Processing and requirements: 1. Serum: room temperature blood is naturally coagulated for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. 2. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen, mixed for 10-20 minutes, and centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 3. Urine: collected in a sterile tube and centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, centrifuge again. Pleural and ascites, cerebrospinal fluid reference implementation. 4. Cell culture supernatant: When detecting secreted components, collect with a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When detecting the components inside the cells, dilute the cell suspension with PBS (PH7.2-7.4), and the cell concentration will reach about 1 million / ml. Through repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 5. Organize the specimen: after cutting the specimen, weigh it. Add a certain amount of PBS, PH7.4. Quickly freeze and save with liquid nitrogen for later use. After the specimen melts, it still maintains a temperature of 2-8 ° C. Add a certain amount of PBS (PH7.4) and homogenize the specimen with a manual or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. After aliquoting, a portion is to be tested, and the rest is frozen for future use. 6. The specimen should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ° C, but repeated freezing and thawing should be avoided. 7. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP). Operating steps Dilution and sample loading of standards: set up 10 wells of the standard on the enzyme-coated plate, add 100 μl of the standard in the first and second wells, and then add the standard in the first and second wells 50μl of diluent, mix well; then take 100μl from the first well and the second well and add them to the third and fourth wells respectively, and then add 50μl of standard diluent to the third and fourth wells respectively, mix well; Then take 50μl each in the third and fourth wells and discard it, then add 50μl each to the fifth and sixth wells, and then add 50ul of the standard dilution solution to the fifth and sixth wells respectively, and mix well; After mixing, take 50μl from the fifth and sixth wells and add them to the seventh and eighth wells respectively. Then add 50μl of the standard dilution solution to the seventh and eighth wells respectively. Take 50μl from the eight wells and add them to the ninth and tenth wells. Then add 50μl of the standard dilution solution to the ninth and tenth wells. After mixing, take 50μl from the ninth and tenth wells and discard. (After dilution, the volume of each well is 50μl, and the concentration is 300ng / L, 200ng / L, 100ng / L, 50ng / L, 25ng / L). Sample addition: set up blank wells (the blank control wells do not add samples and enzyme reagents, the rest of the steps are the same) and the sample wells to be tested. Add 40μl of sample diluent to the test sample well of the enzyme-coated plate, and then add 10μl of the sample to be tested (the final dilution of the sample is 5 times). Add the sample and add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, shake gently to mix. Incubation: Seal the plate with a sealing film and incubate at 37 ° C for 30 minutes. Mixing solution: Dilute 30 times (20 times of 48T) concentrated washing solution with distilled water 30 times (20 times of 48T) and then reserve. Washing: Carefully peel off the sealing film, discard the liquid, spin dry, fill each well with the washing solution, let it stand for 30 seconds, then discard, repeat 5 times and pat dry. Enzyme addition: Add 50μl of enzyme label reagent to each well, except for blank wells. Incubation: The operation is the same as 3. Washing: The operation is the same as 5. Color development: Add 50μl of developer A to each well, then add 50μl of developer B, mix gently, and develop at 37 ° C in the dark for 15 minutes. Termination: Add 50μl of stopper solution to each well to stop the reaction (blue at this time) Li to yellow). Measurement: The absorbance (OD value) of each well was measured in sequence with the blank air conditioner at zero and 450 nm wavelength. The measurement should be carried out within 15 minutes after adding the stop solution. Note: The kit should be taken out of the refrigerated environment and equilibrated at room temperature for 15-30 minutes before use. If the enzyme label coated plate is unopened after opening, the strip should be stored in a sealed bag. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in a water bath during dilution, and the results will not be affected during washing. The sampler should be used at each step of sample addition, and the accuracy should be regularly checked to avoid test errors. It is best to control the sampling time within 5 minutes. If there are many specimens, it is recommended to use a volley gun to add samples. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the test substance in the specimen is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please first dilute it with a certain multiple (n times) of the sample diluent and then determine it. When calculating, please multiply the total dilution Multiple (× n × 5). The sealing film is limited to one-time use to avoid cross-contamination. Please keep the substrate away from light. Carry out the operation in strict accordance with the instructions. The test result must be determined by the reading of the microplate reader. All samples, washing liquid and various wastes should be treated as infectious agents. The components of different batches of this reagent shall not be mixed. 10. If it is different from the English manual, the English manual shall prevail. Calculation: Taking the concentration of the standard as the abscissa and the OD as the ordinate, draw a standard curve on the graph paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or use the standard The linear regression equation of the standard curve is calculated by the concentration and OD value of the sample, the OD value of the sample is substituted into the equation, the sample concentration is calculated, and then multiplied by the dilution factor, which is the actual concentration of the sample. (This picture is for reference only) Kit performance: 1. The linear regression of the sample and the expected concentration correlation coefficient R value is more than 0.92. 2. The batch and batch see should be less than 9% and 15% respectively. Storage conditions and expiration date: 1. Kit storage :; 2-8 ℃. 2. Validity: 6 months